androstenedioneprostaglandin e2galectin-3hyaluronic acidasprosin

Home Human CYCS ELISA Kit

Category

Human CYCS ELISA Kit

Contact Us
  • Alternative name

    Human CYC ELISA Kit;Human HCS ELISA Kit;Human THC4 ELISA Kit;Human cytochrome c, somatic ELISA Kit;Human cytochrome c ELISA Kit;
  • Catalog
    E010950
  • species
    Human
  • GeneCYCS
  • Other Species Mouse Cycs ELISA KitBovine CYCS ELISA KitCanine CYCS ELISA KitHorse CYCS ELISA KitPorcine CYCS ELISA KitRabbit CYCS ELISA KitRat Cycs ELISA KitSheep CYCS ELISA Kit
  • SpecificityThe Human Cytochrome c ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Cytochrome c proteins within the range of 39.1 pg/ml - 2500 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman CYCS ELISA Kit allows for the in vitro quantitative determination of CYCS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Human Cytochrome c ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cytochrome c in Cell Lysates. This assay employs an antibody specific for Human Cytochrome c coated on a 96-well plate. Standards and samples are pipetted into the wells and Cytochrome c present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cytochrome c antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome c bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Human somatic cytochrome c is a 15 kDa, 104 amino acid polypeptide that participates in both oxidative phosphorylation and apoptosis. It exhibits 91% aa identity with mouse/rat cytochrome c. The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c. This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space. Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex. This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes.
  • Human Cytochrome c Protein information
  • Uniprot ID CYC_HUMAN
  • Uniprot AC P99999; A4D166; B2R4I1; P00001; Q6NUR2; Q6NX69; Q96BV4;
  • UniGene Hs.437060; Hs.617193;
  • GeneID 54205
  • KEGG hsa:54205;
  • Human Cytochrome c Protein SEQUENCE

  • SEQUENCE 105 AA; 11749 MW; 8EE9689E0102506B CRC64;

    MGDVEKGKKI FIMKCSQCHT VEKGGKHKTG PNLHGLFGRK TGQAPGYSYT

    AANKNKGIIW GEDTLMEYLE NPKKYIPGTK MIFVGIKKKE ERADLIAYLK

    KATNE

  • UCSC uc003sxl.4; human.;


Related products with Human CYCS ELISA Kit

Inquiry About Human CYCS ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!