Human PGE2 ELISA Kit

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  • Alternative name

    Prostaglandin E2 ELISA Kit

  • Catalog
    E031279
  • species
    Human
  • GenePGE2
  • Standard CurveHuman PGE2 ELISA Kit
  • Other Species Human PGE2 ELISA KitMouse PGE2 ELISA KitBovine PGE2 ELISA KitCanine PGE2 ELISA KitChicken PGE2 ELISA KitGeneral PGE2 ELISA KitPorcine PGE2 ELISA KitRabbit PGE2 ELISA KitRat PGE2 ELISA KitCamel PGE2 ELISA KitGoat PGE2 ELISA KitGuinea Pig PGE2 ELISA KitHamster PGE2 ELISA KitHorse PGE2 ELISA KitMonkey PGE2 ELISA KitSheep PGE2 ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity18.75pg/ml
  • Intended UseHuman PGE2 ELISA Kit allows for the in vitro quantitative determination of PGE2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Human PGE2 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to PGE2 , During the reaction,PGE2 in the sample or standard competes with a fixed amount of biotin-labeled PGE2 for sites on a pre-coated Monoclonal antibody specific to PGE2 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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