Human DHH ELISA Kit

Alternative name
Human DHH ELISA Kit;Human HHG-3 ELISA Kit;Human GDXYM ELISA Kit;Human SRXY7 ELISA Kit;Human desert hedgehog ELISA Kit;Human desert hedgehog protein ELISA Kit;
Catalog
E018090
species
Human
GeneDHH
Standard Curve
Other Species Mouse Dhh ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Desert (DHH). No significant cross-reactivity or interference between Hedgehog Homolog, Desert (DHH) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.064ng/mL.
Intended UseHuman DHH ELISA Kit allows for the in vitro quantitative determination of DHH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Desert (DHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Desert (DHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Desert (DHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Desert (DHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Desert hedgehog protein C-product Protein information
Uniprot ID DHH_HUMAN
Uniprot AC O43323; Q15794;
UniGene Hs.524382; Hs.533776;
GeneID 50846
KEGG hsa:50846;
Human Desert hedgehog protein C-product Protein SEQUENCE
SEQUENCE 396 AA; 43577 MW; FCE4FB21972C3AD5 CRC64;
MALLTNLLPL CCLALLALPA QSCGPGRGPV GRRRYARKQL VPLLYKQFVP
GVPERTLGAS GPAEGRVARG SERFRDLVPN YNPDIIFKDE ENSGADRLMT
ERCKERVNAL AIAVMNMWPG VRLRVTEGWD EDGHHAQDSL HYEGRALDIT
TSDRDRNKYG LLARLAVEAG FDWVYYESRN HVHVSVKADN SLAVRAGGCF
PGNATVRLWS GERKGLRELH RGDWVLAADA SGRVVPTPVL LFLDRDLQRR
ASFVAVETEW PPRKLLLTPW HLVFAARGPA PAPGDFAPVF ARRLRAGDSV
LAPGGDALRP ARVARVAREE AVGVFAPLTA HGTLLVNDVL ASCYAVLESH
QWAHRAFAPL RLLHALGALL PGGAVQPTGM HWYSRLLYRL AEELLG
UCSC uc001rtf.4; human.;

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Category

Human DHH ELISA Kit

Human DHH ELISA Kit;Human HHG-3 ELISA Kit;Human GDXYM ELISA Kit;Human SRXY7 ELISA Kit;Human desert hedgehog ELISA Kit;Human desert hedgehog protein ELISA Kit;