Human DHH ELISA Kit

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  • Alternative name

    Human DHH ELISA Kit;Human HHG-3 ELISA Kit;Human GDXYM ELISA Kit;Human SRXY7 ELISA Kit;Human desert hedgehog ELISA Kit;Human desert hedgehog protein ELISA Kit;

  • Catalog
    E018090
  • species
    Human
  • GeneDHH
  • Standard CurveHuman DHH ELISA Kit
  • Other Species Mouse Dhh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Desert (DHH). No significant cross-reactivity or interference between Hedgehog Homolog, Desert (DHH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman DHH ELISA Kit allows for the in vitro quantitative determination of DHH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Desert (DHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Desert (DHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Desert (DHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Desert (DHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Desert hedgehog protein C-product Protein information
  • Uniprot ID DHH_HUMAN
  • Uniprot AC O43323; Q15794;
  • UniGene Hs.524382; Hs.533776;
  • GeneID 50846
  • KEGG hsa:50846;
  • Human Desert hedgehog protein C-product Protein SEQUENCE
  • SEQUENCE 396 AA; 43577 MW; FCE4FB21972C3AD5 CRC64;

    MALLTNLLPL CCLALLALPA QSCGPGRGPV GRRRYARKQL VPLLYKQFVP

    GVPERTLGAS GPAEGRVARG SERFRDLVPN YNPDIIFKDE ENSGADRLMT

    ERCKERVNAL AIAVMNMWPG VRLRVTEGWD EDGHHAQDSL HYEGRALDIT

    TSDRDRNKYG LLARLAVEAG FDWVYYESRN HVHVSVKADN SLAVRAGGCF

    PGNATVRLWS GERKGLRELH RGDWVLAADA SGRVVPTPVL LFLDRDLQRR

    ASFVAVETEW PPRKLLLTPW HLVFAARGPA PAPGDFAPVF ARRLRAGDSV

    LAPGGDALRP ARVARVAREE AVGVFAPLTA HGTLLVNDVL ASCYAVLESH

    QWAHRAFAPL RLLHALGALL PGGAVQPTGM HWYSRLLYRL AEELLG

  • UCSC uc001rtf.4; human.;


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