Human LGALS3 ELISA Kit

Alternative name
Human Gal-3 ELISA Kit;Human 35 kDa lectin ELISA Kit;Human carbohydrate-binding protein 35 ELISA Kit;Human CBP 35 ELISA Kit;Human galactose-specific lectin 3 ELISA Kit;Human Galactoside-binding protein ELISA Kit;Human GALBP ELISA Kit;Human IgE-binding protein ELISA Kit;Human L-31 ELISA Kit;Human laminin-binding protein ELISA Kit;Human lectin L-29 ELISA Kit;Human MAC-2 antigen ELISA Kit;Human MAC2 ELISA Kit;Human CBP35 ELISA Kit;Human GAL3 ELISA Kit;Human GALIG ELISA Kit;Human L31 ELISA Kit;Human LGALS2 ELISA Kit;Human galectin 3 ELISA Kit;Human galectin-3 ELISA Kit;Human advanced glycation end-product receptor 3 ELISA Kit;Human lectin, galactoside-binding, soluble, 3 ELISA Kit;
Catalog
E015982
species
Human
GeneLGALS3
Standard Curve
Other Species Mouse Lgals3 ELISA Kit Canine LGALS3 ELISA Kit Rabbit LGALS3 ELISA Kit Rat Lgals3 ELISA Kit
SpecificityThe Human Galectin 3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Galectin 3 proteins within the range of 156 pg/ml - 10000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Human Galectin 3 is typically less than 10 pg/ml.
Intended UseHuman LGALS3 ELISA Kit allows for the in vitro quantitative determination of LGALS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The Human Galectin 3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Galectin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Human Galectin-3 Protein information
Uniprot ID LEG3_HUMAN
Uniprot AC P17931; B2RC38; Q16005; Q6IBA7; Q96J47;
UniGene Hs.531081;
GeneID 3958
KEGG hsa:3958;
Human Galectin-3 Protein SEQUENCE
SEQUENCE 250 AA; 26152 MW; C49DDF6D67AE0C88 CRC64;
MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP
PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA
YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ
RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI
QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
UCSC uc001xbr.4; human.;

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Human LGALS3 ELISA Kit