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Human HA ELISA Kit

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  • Alternative name

    Lymphatic vessel endothelial hyaluronic acid receptor 1 ELISA KIT; Cell surface retention sequence-binding protein 1 ELISA KIT; CRSBP-1 ELISA KIT; Extracellular link domain-containing protein 1 ELISA KIT; Hyaluronic acid receptorLYVE1 ELISA KIT; CRSBP1 ELISA KIT; HAR ELISA KIT; XLKD1 ELISA KIT; UNQ230/PRO263 ELISA KIT; LYVE-1 ELISA KIT; CRSBP-1 ELISA KIT
  • Catalog
    E019081
  • species
    Human
  • GeneHA
  • Standard CurveHuman HA ELISA Kit
  • Other Species Human HA ELISA KitHuman F-H1N1-HA ELISA KitHuman F-H5N1-HA ELISA KitMouse HA ELISA KitMouse F-H1N1-HA ELISA KitMouse F-H3N2-HA ELISA KitMouse F-H5N1-HA ELISA KitMouse F-H7N7-HA ELISA KitBovine HA ELISA KitCanine HA ELISA KitChicken HA ELISA KitGeneral HA ELISA KitPorcine HA ELISA KitRabbit HA ELISA KitRat HA ELISA KitCamel HA ELISA KitGoat HA ELISA KitGuinea Pig HA ELISA KitHamster HA ELISA KitHorse HA ELISA KitMonkey HA ELISA KitSheep HA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HA. No significant cross-reactivity or interference between HA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HA ELISA Kit allows for the in vitro quantitative determination of HA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Intended Uses: This HA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HA antibody and an HA-HRP conjugate. The assay sample and buffer are incubated together with HA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HA concentration since HA from samples and HA-HRP conjugate compete for the anti-HA antibody binding site. Since the number of sites is limited, as more sites are occupied by HA from the sample, fewer sites are left to bind HA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.


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