SpecificityThis assay has high sensitivity and excellent specificity for detection of 3alphaAS. No significant cross-reactivity or interference between 3alphaAS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 3alphaAS and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman 3Alphadiol ELISA Kit allows for the in vitro quantitative determination of 3Alphadiol , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: 3alphaAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-3alphaAS antibody and an 3alphaAS-HRP conjugate. The assay sample and buffer are incubated together with 3alphaAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 3alphaAS concentration since 3alphaAS from samples and 3alphaAS-HRP conjugate compete for the anti-3alphaAS antibody binding site. Since the number of sites is limited, as more sites are occupied by 3alphaAS from the sample, fewer sites are left to bind 3alphaAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3alphaAS concentration in each sample is interpolated from this standard curve.