Human CSPG4 ELISA Kit

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  • Alternative name

    Human chondroitin sulfate proteoglycan NG2 ELISA Kit;Human melanoma chondroitin sulfate proteoglycan ELISA Kit;Human melanoma-associated chondroitin sulfate proteoglycan ELISA Kit;Human MCSP ELISA Kit;Human HMW-MAA ELISA Kit;Human MCSPG ELISA Kit;Human MEL-CSPG ELISA Kit;Human MSK16 ELISA Kit;Human NG2 ELISA Kit;Human chondroitin sulfate proteoglycan 4 ELISA Kit;Human chondroitin sulfate proteoglycan 4 (melanoma-associated) ELISA Kit;

  • Catalog
    E008318
  • species
    Human
  • GeneCSPG4
  • Standard CurveHuman CSPG4 ELISA Kit
  • Other Species Mouse Cspg4 ELISA KitRat Cspg4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CSPG4. No significant cross-reactivity or interference between CSPG4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSPG4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CSPG4 ELISA Kit allows for the in vitro quantitative determination of CSPG4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CSPG4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSPG4 antibody and an CSPG4-HRP conjugate. The assay sample and buffer are incubated together with CSPG4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSPG4 concentration since CSPG4 from samples and CSPG4-HRP conjugate compete for the anti-CSPG4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSPG4 from the sample, fewer sites are left to bind CSPG4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSPG4 concentration in each sample is interpolated from this standard curve.
  • Human Chondroitin sulfate proteoglycan 4 Protein information
  • Uniprot ID CSPG4_HUMAN
  • Uniprot AC Q6UVK1; D3DW77; Q92675;
  • UniGene Hs.513044;
  • GeneID 1464
  • KEGG hsa:1464;
  • Human Chondroitin sulfate proteoglycan 4 Protein SEQUENCE
  • SEQUENCE 2322 AA; 250537 MW; 0B4F39AFC5ADD3CA CRC64;

    MQSGPRPPLP APGLALALTL TMLARLASAA SFFGENHLEV PVATALTDID

    LQLQFSTSQP EALLLLAAGP ADHLLLQLYS GRLQVRLVLG QEELRLQTPA

    ETLLSDSIPH TVVLTVVEGW ATLSVDGFLN ASSAVPGAPL EVPYGLFVGG

    TGTLGLPYLR GTSRPLRGCL HAATLNGRSL LRPLTPDVHE GCAEEFSASD

    DVALGFSGPH SLAAFPAWGT QDEGTLEFTL TTQSRQAPLA FQAGGRRGDF

    IYVDIFEGHL RAVVEKGQGT VLLHNSVPVA DGQPHEVSVH INAHRLEISV

    DQYPTHTSNR GVLSYLEPRG SLLLGGLDAE ASRHLQEHRL GLTPEATNAS

    LLGCMEDLSV NGQRRGLREA LLTRNMAAGC RLEEEEYEDD AYGHYEAFST

    LAPEAWPAME LPEPCVPEPG LPPVFANFTQ LLTISPLVVA EGGTAWLEWR

    HVQPTLDLME AELRKSQVLF SVTRGARHGE LELDIPGAQA RKMFTLLDVV

    NRKARFIHDG SEDTSDQLVL EVSVTARVPM PSCLRRGQTY LLPIQVNPVN

    DPPHIIFPHG SLMVILEHTQ KPLGPEVFQA YDPDSACEGL TFQVLGTSSG

    LPVERRDQPG EPATEFSCRE LEAGSLVYVH RGGPAQDLTF RVSDGLQASP

    PATLKVVAIR PAIQIHRSTG LRLAQGSAMP ILPANLSVET NAVGQDVSVL

    FRVTGALQFG ELQKQGAGGV EGAEWWATQA FHQRDVEQGR VRYLSTDPQH

    HAYDTVENLA LEVQVGQEIL SNLSFPVTIQ RATVWMLRLE PLHTQNTQQE

    TLTTAHLEAT LEEAGPSPPT FHYEVVQAPR KGNLQLQGTR LSDGQGFTQD

    DIQAGRVTYG ATARASEAVE DTFRFRVTAP PYFSPLYTFP IHIGGDPDAP

    VLTNVLLVVP EGGEGVLSAD HLFVKSLNSA SYLYEVMERP RHGRLAWRGT

    QDKTTMVTSF TNEDLLRGRL VYQHDDSETT EDDIPFVATR QGESSGDMAW

    EEVRGVFRVA IQPVNDHAPV QTISRIFHVA RGGRRLLTTD DVAFSDADSG

    FADAQLVLTR KDLLFGSIVA VDEPTRPIYR FTQEDLRKRR VLFVHSGADR

    GWIQLQVSDG QHQATALLEV QASEPYLRVA NGSSLVVPQG GQGTIDTAVL

    HLDTNLDIRS GDEVHYHVTA GPRWGQLVRA GQPATAFSQQ DLLDGAVLYS

    HNGSLSPRDT MAFSVEAGPV HTDATLQVTI ALEGPLAPLK LVRHKKIYVF

    QGEAAEIRRD QLEAAQEAVP PADIVFSVKS PPSAGYLVMV SRGALADEPP

    SLDPVQSFSQ EAVDTGRVLY LHSRPEAWSD AFSLDVASGL GAPLEGVLVE

    LEVLPAAIPL EAQNFSVPEG GSLTLAPPLL RVSGPYFPTL LGLSLQVLEP

    PQHGALQKED GPQARTLSAF SWRMVEEQLI RYVHDGSETL TDSFVLMANA

    SEMDRQSHPV AFTVTVLPVN DQPPILTTNT GLQMWEGATA PIPAEALRST

    DGDSGSEDLV YTIEQPSNGR VVLRGAPGTE VRSFTQAQLD GGLVLFSHRG

    TLDGGFRFRL SDGEHTSPGH FFRVTAQKQV LLSLKGSQTL TVCPGSVQPL

    SSQTLRASSS AGTDPQLLLY RVVRGPQLGR LFHAQQDSTG EALVNFTQAE

    VYAGNILYEH EMPPEPFWEA HDTLELQLSS PPARDVAATL AVAVSFEAAC

    PQRPSHLWKN KGLWVPEGQR ARITVAALDA SNLLASVPSP QRSEHDVLFQ

    VTQFPSRGQL LVSEEPLHAG QPHFLQSQLA AGQLVYAHGG GGTQQDGFHF

    RAHLQGPAGA SVAGPQTSEA FAITVRDVNE RPPQPQASVP LRLTRGSRAP

    ISRAQLSVVD PDSAPGEIEY EVQRAPHNGF LSLVGGGLGP VTRFTQADVD

    SGRLAFVANG SSVAGIFQLS MSDGASPPLP MSLAVDILPS AIEVQLRAPL

    EVPQALGRSS LSQQQLRVVS DREEPEAAYR LIQGPQYGHL LVGGRPTSAF

    SQFQIDQGEV VFAFTNFSSS HDHFRVLALA RGVNASAVVN VTVRALLHVW

    AGGPWPQGAT LRLDPTVLDA GELANRTGSV PRFRLLEGPR HGRVVRVPRA

    RTEPGGSQLV EQFTQQDLED GRLGLEVGRP EGRAPGPAGD SLTLELWAQG

    VPPAVASLDF ATEPYNAARP YSVALLSVPE AARTEAGKPE SSTPTGEPGP

    MASSPEPAVA KGGFLSFLEA NMFSVIIPMC LVLLLLALIL PLLFYLRKRN

    KTGKHDVQVL TAKPRNGLAG DTETFRKVEP GQAIPLTAVP GQGPPPGGQP

    DPELLQFCRT PNPALKNGQY WV

  • UCSC uc002baw.3; human.;


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