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Alternative name
Gamma-tubulin complex component 2 ELISA KIT; Gamma-ring complex protein 103 kDa ELISA KIT; h103p ELISA KIT; hGrip103 ELISA KIT; Spindle pole body protein Spc97 homolog ELISA KIT; hSpc97TUBGCP2 ELISA KIT; GCP2 ELISA KIT; GCP-2 ELISA KIT; hGCP2 ELISA KIT; h103p ELISA KIT; hGrip103 ELISA KIT; hSpc97 ELISA KIT
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Catalog
E017393
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species
Human
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GeneGCP2
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman GCP2 ELISA Kit allows for the in vitro quantitative determination of GCP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalPrinciple of the Assay: GCP2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GCP2. Standards or samples are then added to the microtiter plate wells and GCP2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GCP2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GCP2 are added to each well to "sandwich" the GCP2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GCP2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCP2 concentration in each sample is interpolated from this standard curve.
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