SpecificityThis assay has high sensitivity and excellent specificity for detection of Inhibin B (INHB).
No significant cross-reactivity or interference between Inhibin B (INHB) and analogues was observed.
Intended UseHuman INHB ELISA Kit allows for the in vitro quantitative determination of INHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin B (INHB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin B (INHB) and unlabeled Inhibin B (INHB) (Standards or samples) with the pre-coated antibody specific to Inhibin B (INHB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin B (INHB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin B (INHB) in the sample.