Human PPARA ELISA Kit

Alternative name
Human PPAR-alpha ELISA Kit;Human nuclear receptor subfamily 1 group C member 1 ELISA Kit;Human NR1C1 ELISA Kit;Human PPAR ELISA Kit;Human PPARalpha ELISA Kit;Human hPPAR ELISA Kit;Human peroxisome proliferator activated receptor alpha ELISA Kit;Human peroxisome proliferator-activated receptor alpha ELISA Kit;Human peroxisome proliferative activated receptor, alpha ELISA Kit;Human peroxisome proliferator-activated nuclear receptor alpha variant 3 ELISA Kit;
Catalog
E028741
species
Human
GenePPARA
Standard Curve
Other Species Mouse Ppara ELISA Kit Canine PPARA ELISA Kit Guinea Pig PPARA ELISA Kit Rat Ppara ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Alpha (PPARa). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Alpha (PPARa) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.112ng/mL.
Intended UseHuman PPARA ELISA Kit allows for the in vitro quantitative determination of PPARA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Peroxisome proliferator-activated receptor alpha Protein information
Uniprot ID PPARA_HUMAN
Uniprot AC Q07869; B0G0X3; Q16241; Q6I9S0; Q92486; Q92689; Q9Y3N1;
UniGene Hs.103110; Hs.710044;
GeneID 5465
KEGG hsa:5465;
Human Peroxisome proliferator-activated receptor alpha Protein SEQUENCE
SEQUENCE 468 AA; 52225 MW; 850846FD51ADA883 CRC64;
MVDTESPLCP LSPLEAGDLE SPLSEEFLQE MGNIQEISQS IGEDSSGSFG
FTEYQYLGSC PGSDGSVITD TLSPASSPSS VTYPVVPGSV DESPSGALNI
ECRICGDKAS GYHYGVHACE GCKGFFRRTI RLKLVYDKCD RSCKIQKKNR
NKCQYCRFHK CLSVGMSHNA IRFGRMPRSE KAKLKAEILT CEHDIEDSET
ADLKSLAKRI YEAYLKNFNM NKVKARVILS GKASNNPPFV IHDMETLCMA
EKTLVAKLVA NGIQNKEAEV RIFHCCQCTS VETVTELTEF AKAIPGFANL
DLNDQVTLLK YGVYEAIFAM LSSVMNKDGM LVAYGNGFIT REFLKSLRKP
FCDIMEPKFD FAMKFNALEL DDSDISLFVA AIICCGDRPG LLNVGHIEKM
QEGIVHVLRL HLQSNHPDDI FLFPKLLQKM ADLRQLVTEH AQLVQIIKKT
ESDAALHPLL QEIYRDMY
UCSC uc003bgx.1; human. [Q07869-1];

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