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Human GRIA4 ELISA Kit

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  • Alternative name

    Human gluR-4 ELISA Kit;Human GLUR4 ELISA Kit;Human AMPA-selective glutamate receptor 4 ELISA Kit;Human gluR-D ELISA Kit;Human Glutamate receptor ionotropic, AMPA 4 ELISA Kit;Human GluA4 ELISA Kit;Human GLUR4C ELISA Kit;Human GLURD ELISA Kit;Human glutamate ionotropic receptor AMPA type subunit 4 ELISA Kit;Human glutamate receptor 4 ELISA Kit;Human glutamate receptor, ionotrophic, AMPA 4 ELISA Kit;Human glutamate receptor, ionotropic, AMPA 4 ELISA Kit;
  • Catalog
    E016737
  • species
    Human
  • GeneGRIA4
  • Standard CurveHuman GRIA4 ELISA Kit
  • Other Species Mouse Gria4 ELISA KitMonkey GRIA4 ELISA KitRat Gria4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GRIA4. No significant cross-reactivity or interference between GRIA4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRIA4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GRIA4 ELISA Kit allows for the in vitro quantitative determination of GRIA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical    Intended Uses: This GRIA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRIA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GRIA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRIA4 antibody and an GRIA4-HRP conjugate. The assay sample and buffer are incubated together with GRIA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRIA4 concentration since GRIA4 from samples and GRIA4-HRP conjugate compete for the anti-GRIA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRIA4 from the sample, fewer sites are left to bind GRIA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRIA4 concentration in each sample is interpolated from this standard curve.
  • Human Glutamate receptor 4 Protein information
  • Uniprot ID GRIA4_HUMAN
  • Uniprot AC P48058; Q86XE8;
  • UniGene Hs.503743;
  • GeneID 2893
  • KEGG hsa:2893;
  • Human Glutamate receptor 4 Protein SEQUENCE

  • SEQUENCE 902 AA; 100871 MW; 7793AEF0AB829FC5 CRC64;

    MRIISRQIVL LFSGFWGLAM GAFPSSVQIG GLFIRNTDQE YTAFRLAIFL

    HNTSPNASEA PFNLVPHVDN IETANSFAVT NAFCSQYSRG VFAIFGLYDK

    RSVHTLTSFC SALHISLITP SFPTEGESQF VLQLRPSLRG ALLSLLDHYE

    WNCFVFLYDT DRGYSILQAI MEKAGQNGWH VSAICVENFN DVSYRQLLEE

    LDRRQEKKFV IDCEIERLQN ILEQIVSVGK HVKGYHYIIA NLGFKDISLE

    RFIHGGANVT GFQLVDFNTP MVIKLMDRWK KLDQREYPGS ETPPKYTSAL

    TYDGVLVMAE TFRSLRRQKI DISRRGNAGD CLANPAAPWG QGIDMERTLK

    QVRIQGLTGN VQFDHYGRRV NYTMDVFELK STGPRKVGYW NDMDKLVLIQ

    DVPTLGNDTA AIENRTVVVT TIMESPYVMY KKNHEMFEGN DKYEGYCVDL

    ASEIAKHIGI KYKIAIVPDG KYGARDADTK IWNGMVGELV YGKAEIAIAP

    LTITLVREEV IDFSKPFMSL GISIMIKKPQ KSKPGVFSFL DPLAYEIWMC

    IVFAYIGVSV VLFLVSRFSP YEWHTEEPED GKEGPSDQPP NEFGIFNSLW

    FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA NLAAFLTVER

    MVSPIESAED LAKQTEIAYG TLDSGSTKEF FRRSKIAVYE KMWTYMRSAE

    PSVFTRTTAE GVARVRKSKG KFAFLLESTM NEYIEQRKPC DTMKVGGNLD

    SKGYGVATPK GSSLRTPVNL AVLKLSEAGV LDKLKNKWWY DKGECGPKDS

    GSKDKTSALS LSNVAGVFYI LVGGLGLAML VALIEFCYKS RAEAKRMKLT

    FSEAIRNKAR LSITGSVGEN GRVLTPDCPK AVHTGTAIRQ SSGLAVIASD

    LP

  • UCSC uc001piu.2; human. [P48058-1];


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