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Human CYP7A1 ELISA Kit

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  • Alternative name

    Human CYPVII ELISA Kit;Human cholesterol 7-alpha-hydroxylase ELISA Kit;Human cytochrome P450 7A1 ELISA Kit;Human CYP7 ELISA Kit;Human CP7A ELISA Kit;Human cytochrome P450 family 7 subfamily A member 1 ELISA Kit;Human cholesterol 7-alpha-monooxygenase ELISA Kit;Human cytochrome P450, family 7, subfamily A, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily VIIA polypeptide 1 ELISA Kit;
  • Catalog
    E011127
  • species
    Human
  • GeneCYP7A1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CYP7A1. No significant cross-reactivity or interference between Human CYP7A1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity4.7 pg/ml
  • Intended UseHuman CYP7A1 ELISA Kit allows for the in vitro quantitative determination of CYP7A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Cholesterol 7-alpha-monooxygenase Protein information
  • Uniprot ID CP7A1_HUMAN
  • Uniprot AC P22680; P78454; Q3MIL8; Q7KZ19;
  • UniGene Hs.1644;
  • GeneID 1581
  • KEGG hsa:1581;
  • Human Cholesterol 7-alpha-monooxygenase Protein SEQUENCE

  • SEQUENCE 504 AA; 57661 MW; D8067E0FF6342949 CRC64;

    MMTTSLIWGI AIAACCCLWL ILGIRRRQTG EPPLENGLIP YLGCALQFGA

    NPLEFLRANQ RKHGHVFTCK LMGKYVHFIT NPLSYHKVLC HGKYFDWKKF

    HFATSAKAFG HRSIDPMDGN TTENINDTFI KTLQGHALNS LTESMMENLQ

    RIMRPPVSSN SKTAAWVTEG MYSFCYRVMF EAGYLTIFGR DLTRRDTQKA

    HILNNLDNFK QFDKVFPALV AGLPIHMFRT AHNAREKLAE SLRHENLQKR

    ESISELISLR MFLNDTLSTF DDLEKAKTHL VVLWASQANT IPATFWSLFQ

    MIRNPEAMKA ATEEVKRTLE NAGQKVSLEG NPICLSQAEL NDLPVLDSII

    KESLRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI

    YPDPLTFKYD RYLDENGKTK TTFYCNGLKL KYYYMPFGSG ATICPGRLFA

    IHEIKQFLIL MLSYFELELI EGQAKCPPLD QSRAGLGILP PLNDIEFKYK

    FKHL

  • UCSC uc003xtm.5; human.;



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