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Human CTACK ELISA Kit

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  • Alternative name

    C-C motif chemokine 27 ELISA KIT; CC chemokine ILC ELISA KIT; Cutaneous T-cell-attracting chemokine ELISA KIT; CTACK ELISA KIT; ESkine ELISA KIT; IL-11 R-alpha-locus chemokine ELISA KIT; Skinkine ELISA KIT; Small-inducible cytokine A27CCL27 ELISA KIT; ILC ELISA KIT; SCYA27 ELISA KIT; CTACK ELISA KIT
  • Catalog
    E010402
  • species
    Human
  • GeneCTACK
  • Standard CurveHuman CTACK ELISA Kit
  • Other Species Human CTACK/CCL27 ELISA KitMouse CTACK ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CTACK ELISA Kit allows for the in vitro quantitative determination of CTACK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the Assay: CTACK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CTACK. Standards or samples are then added to the microtiter plate wells and CTACK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CTACK present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CTACK are added to each well to "sandwich" the CTACK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CTACK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTACK concentration in each sample is interpolated from this standard curve.


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