Intended UseHuman PHLDA2 ELISA Kit allows for the in vitro quantitative determination of PHLDA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description specificalPrinciple of the Assay: PHLDA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PHLDA2. Standards or samples are then added to the microtiter plate wells and PHLDA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PHLDA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PHLDA2 are added to each well to "sandwich" the PHLDA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PHLDA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHLDA2 concentration in each sample is interpolated from this standard curve.
Human Pleckstrin homology-like domain family A member 2 Protein information
Uniprot ID
PHLA2_HUMAN
Uniprot AC
Q53GA4; O00496;
UniGene
Hs.154036;
GeneID
7262
KEGG
hsa:7262;
Human Pleckstrin homology-like domain family A member 2 Protein SEQUENCE