Human CRY1 ELISA Kit

Alternative name
Human PHLL1 ELISA Kit;Human cryptochrome circadian clock 1 ELISA Kit;Human cryptochrome-1 ELISA Kit;Human cryptochrome 1 (photolyase-like) ELISA Kit;
Catalog
E010274
species
Human
GeneCRY1
Standard Curve
Other Species Mouse Cry1 ELISA Kit Chicken CRY1 ELISA Kit Monkey CRY1 ELISA Kit Rat Cry1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CRY1 ELISA Kit allows for the in vitro quantitative determination of CRY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: CRY1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRY1. Standards or samples are then added to the microtiter plate wells and CRY1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRY1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CRY1 are added to each well to "sandwich" the CRY1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRY1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRY1 concentration in each sample is interpolated from this standard curve.
Human Cryptochrome-1 Protein information
Uniprot ID CRY1_HUMAN
Uniprot AC Q16526;
UniGene Hs.151573;
GeneID 1407
KEGG hsa:1407;
Human Cryptochrome-1 Protein SEQUENCE
SEQUENCE 586 AA; 66395 MW; 96A5B09A6364D3B9 CRC64;
MGVNAVHWFR KGLRLHDNPA LKECIQGADT IRCVYILDPW FAGSSNVGIN
RWRFLLQCLE DLDANLRKLN SRLFVIRGQP ADVFPRLFKE WNITKLSIEY
DSEPFGKERD AAIKKLATEA GVEVIVRISH TLYDLDKIIE LNGGQPPLTY
KRFQTLISKM EPLEIPVETI TSEVIEKCTT PLSDDHDEKY GVPSLEELGF
DTDGLSSAVW PGGETEALTR LERHLERKAW VANFERPRMN ANSLLASPTG
LSPYLRFGCL SCRLFYFKLT DLYKKVKKNS SPPLSLYGQL LWREFFYTAA
TNNPRFDKME GNPICVQIPW DKNPEALAKW AEGRTGFPWI DAIMTQLRQE
GWIHHLARHA VACFLTRGDL WISWEEGMKV FEELLLDADW SINAGSWMWL
SCSSFFQQFF HCYCPVGFGR RTDPNGDYIR RYLPVLRGFP AKYIYDPWNA
PEGIQKVAKC LIGVNYPKPM VNHAEASRLN IERMKQIYQQ LSRYRGLGLL
ASVPSNPNGN GGFMGYSAEN IPGCSSSGSC SQGSGILHYA HGDSQQTHLL
KQGRSSMGTG LSGGKRPSQE EDTQSIGPKV QRQSTN
UCSC uc001tmi.5; human.;

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Category

Human CRY1 ELISA Kit

Human PHLL1 ELISA Kit;Human cryptochrome circadian clock 1 ELISA Kit;Human cryptochrome-1 ELISA Kit;Human cryptochrome 1 (photolyase-like) ELISA Kit;