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Alternative name
Vascular endothelial growth factor C ELISA KIT; Flt4 ligand ELISA KIT; Flt4-L ELISA KIT; Vascular endothelial growth factor-related protein ELISA KIT; VRPVEGFC ELISA KIT; VEGF-C ELISA KIT; Flt4-L ELISA KIT; VRP ELISA KIT
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Catalog
E072364
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species
Mouse
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GeneVEGF.C
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Standard Curve
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Other Species
Mouse VEGF-C ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse VEGF.C ELISA Kit allows for the in vitro quantitative determination of VEGF.C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCancer
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Product Description
specificalPrinciple of the Assay: VEGF.C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VEGF.C antibody and an VEGF.C-HRP conjugate. The assay sample and buffer are incubated together with VEGF.C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF.C concentration since VEGF.C from samples and VEGF.C-HRP conjugate compete for the anti-VEGF.C antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF.C from the sample, fewer sites are left to bind VEGF.C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF.C concentration in each sample is interpolated from this standard curve.
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