Human ANXA2 ELISA Kit

Alternative name
Human annexin II ELISA Kit;Human annexin-2 ELISA Kit;Human calpactin I heavy chain ELISA Kit;Human calpactin-1 heavy chain ELISA Kit;Human Chromobindin-8 ELISA Kit;Human lipocortin II ELISA Kit;Human placental anticoagulant protein IV ELISA Kit;Human PAP-IV ELISA Kit;Human protein I ELISA Kit;Human P36 ELISA Kit;Human ANX2 ELISA Kit;Human ANX2L4 ELISA Kit;Human CAL1H ELISA Kit;Human LPC2D ELISA Kit;Human HEL-S-270 ELISA Kit;Human LIP2 ELISA Kit;Human LPC2 ELISA Kit;Human annexin A2 ELISA Kit;Human calpactin I heavy polypeptide ELISA Kit;Human chromobindin 8 ELISA Kit;Human epididymis secretory protein Li 270 ELISA Kit;
Catalog
E002403
species
Human
GeneANXA2
Standard Curve
Other Species Mouse Anxa2 ELISA Kit Bovine ANXA2 ELISA Kit Canine ANXA2 ELISA Kit Chicken ANXA2 ELISA Kit Porcine ANXA2 ELISA Kit Rat Anxa2 ELISA Kit Sheep ANXA2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ANXA2. No significant cross-reactivity or interference between ANXA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman ANXA2 ELISA Kit allows for the in vitro quantitative determination of ANXA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses||This ANXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ANXA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA2 antibody and an ANXA2-HRP conjugate. The assay sample and buffer are incubated together with ANXA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA2 concentration since ANXA2 from samples and ANXA2-HRP conjugate compete for the anti-ANXA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA2 from the sample, fewer sites are left to bind ANXA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA2 concentration in each sample is interpolated from this standard curve.
Human Annexin A2 Protein information
Uniprot ID ANXA2_HUMAN
Uniprot AC P07355; Q567R4; Q6N0B3; Q8TBV2; Q96DD5; Q9UDH8;
UniGene Hs.511605;
GeneID 302
KEGG hsa:302;
Human Annexin A2 Protein SEQUENCE
SEQUENCE 339 AA; 38604 MW; 5126E1337A0CBEA1 CRC64;
MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG
VDEVTIVNIL TNRSNAQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
YKTDLEKDII SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARDLYD
AGVKRKGTDV PKWISIMTER SVPHLQKVFD RYKSYSPYDM LESIRKEVKG
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM
LKIRSEFKRK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD
UCSC uc002agk.4; human. [P07355-1];

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Human ANXA2 ELISA Kit