Human HLA-DRA ELISA Kit

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  • Alternative name

    Human MHC class II antigen DRA ELISA Kit;Human HLA-DRA1 ELISA Kit;Human major histocompatibility complex, class II, DR alpha ELISA Kit;Human HLA class II histocompatibility antigen, DR alpha chain ELISA Kit;Human histocompatibility antigen HLA-DR alpha ELISA Kit;

  • Catalog
    E018862
  • species
    Human
  • GeneHLA-DRA
  • Standard CurveHuman HLA-DRA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human HLA-DRA. No significant cross-reactivity or interference between human HLA-DRA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HLA-DRA is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman HLA-DRA ELISA Kit allows for the in vitro quantitative determination of HLA-DRA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HLA-DRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HLA-DRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HLA-DRA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HLA-DRA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human HLA class II histocompatibility antigen, DR alpha chain Protein information
  • Uniprot ID DRA_HUMAN
  • Uniprot AC P01903; A2BET4; Q30160; Q6IAZ1; Q861I2; Q9TP70;
  • UniGene Hs.520048;
  • GeneID 3122
  • KEGG hsa:3122;
  • Human HLA class II histocompatibility antigen, DR alpha chain Protein SEQUENCE
  • SEQUENCE 254 AA; 28607 MW; 3CD1CDBA952B2350 CRC64;

    MAISGVPVLG FFIIAVLMSA QESWAIKEEH VIIQAEFYLN PDQSGEFMFD

    FDGDEIFHVD MAKKETVWRL EEFGRFASFE AQGALANIAV DKANLEIMTK

    RSNYTPITNV PPEVTVLTNS PVELREPNVL ICFIDKFTPP VVNVTWLRNG

    KPVTTGVSET VFLPREDHLF RKFHYLPFLP STEDVYDCRV EHWGLDEPLL

    KHWEFDAPSP LPETTENVVC ALGLTVGLVG IIIGTIFIIK GVRKSNAAER

    RGPL

  • UCSC uc003obh.5; human.;



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