Human BLNK ELISA Kit

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  • Alternative name

    Human B-cell adapter containing a SH2 domain protein ELISA Kit;Human B-cell adapter containing a Src homology 2 domain protein ELISA Kit;Human cytoplasmic adapter protein ELISA Kit;Human Src homology 2 domain-containing leukocyte protein of 65 kDa ELISA Kit;Human SLP-65 ELISA Kit;Human BASH ELISA Kit;Human SLP65 ELISA Kit;Human AGM4 ELISA Kit;Human BLNK-S ELISA Kit;Human LY57 ELISA Kit;Human bca ELISA Kit;Human B-cell linker ELISA Kit;Human B-cell linker protein ELISA Kit;Human B cell adaptor containing SH2 domain ELISA Kit;Human B-cell activation ELISA Kit;Human Src homology [SH2] domain-containing leukocyte protein of 65 kD ELISA Kit;

  • Catalog
    E005026
  • species
    Human
  • GeneBLNK
  • Other Species Mouse Blnk ELISA KitChicken BLNK ELISA KitRat Blnk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BLNK. No significant cross-reactivity or interference between Human BLNK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human BLNK and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman BLNK ELISA Kit allows for the in vitro quantitative determination of BLNK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BLNK in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BLNK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human BLNK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BLNK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human BLNK. You can calculate the concentration of Human BLNK in the samples by comparing the OD of the samples to the standard curve.
  • Human B-cell linker protein Protein information
  • Uniprot ID BLNK_HUMAN
  • Uniprot AC Q8WV28; O75498; O75499; Q2MD49;
  • UniGene Hs.665244;
  • GeneID 29760
  • KEGG hsa:29760;
  • Human B-cell linker protein Protein SEQUENCE
  • SEQUENCE 456 AA; 50466 MW; 95F1D5485D03D397 CRC64;

    MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR

    DYASESPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP

    VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT

    STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP

    EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK

    KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ

    KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA

    CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF

    IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK

    YAVKVS

  • UCSC uc001kls.5; human. [Q8WV28-1];


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