IHCImmunohistochemistry of paraffin-embedded human skin using A007501(RRP8 antibody) at dilution of 1:100
Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A007501(RRP8 antibody) at dilution of 1:1500
Recommended dilutionWB : 1:500-1:5000
Product Description specificalEssential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.