Human DNASE1 ELISA Kit

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  • Alternative name

    Human deoxyribonuclease I ELISA Kit;Human DNase I ELISA Kit;Human DNL1 ELISA Kit;Human DRNI ELISA Kit;Human deoxyribonuclease 1 ELISA Kit;Human deoxyribonuclease-1 ELISA Kit;Human DNase I, lysosomal ELISA Kit;Human Dornase alfa ELISA Kit;Human human urine deoxyribonuclease I ELISA Kit;

  • Catalog
    E011697
  • species
    Human
  • GeneDNASE1
  • Standard CurveHuman DNASE1 ELISA Kit
  • Other Species Mouse Dnase1 ELISA KitBovine DNASE1 ELISA KitCanine DNASE1 ELISA KitChicken DNASE1 ELISA KitHorse DNASE1 ELISA KitPorcine DNASE1 ELISA KitRabbit DNASE1 ELISA KitRat Dnase1 ELISA KitSheep DNASE1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Deoxyribonuclease I (DNASE1). No significant cross-reactivity or interference between Deoxyribonuclease I (DNASE1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 34pg/mL.
  • Intended UseHuman DNASE1 ELISA Kit allows for the in vitro quantitative determination of DNASE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease I (DNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxyribonuclease I (DNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxyribonuclease I (DNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxyribonuclease I (DNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Deoxyribonuclease-1 Protein information
  • Uniprot ID DNAS1_HUMAN
  • Uniprot AC P24855; B4DV35; Q14UU9; Q14UV0;
  • UniGene Hs.629638; Hs.733045;
  • GeneID 1773
  • KEGG hsa:1773;
  • Human Deoxyribonuclease-1 Protein SEQUENCE
  • SEQUENCE 282 AA; 31434 MW; 040042E2D23555B6 CRC64;

    MRGMKLLGAL LALAALLQGA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI

    LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE

    RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF

    AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP

    SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD

    SALPFNFQAA YGLSDQLAQA ISDHYPVEVM LK

  • UCSC uc002cvr.4; human. [P24855-1];


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