Human IL18 ELISA Kit

Alternative name
Human IL-18 ELISA Kit;Human iboctadekin ELISA Kit;Human Interferon gamma-inducing factor ELISA Kit;Human IFN-gamma-inducing factor ELISA Kit;Human interleukin-1 gamma ELISA Kit;Human IL-1 gamma ELISA Kit;Human IGIF ELISA Kit;Human IL1F4 ELISA Kit;Human IL-1g ELISA Kit;Human interleukin 18 ELISA Kit;Human interleukin-18 ELISA Kit;Human interleukin 18 (interferon-gamma-inducing factor) ELISA Kit;
Catalog
E019393
species
Human
GeneIL18
SpecificityNatural and recombinant human IL-18
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman IL18 ELISA Kit allows for the in vitro quantitative determination of IL18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, Y33-D197) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18 amount of sample captured in plate. Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
Human Interleukin-18 Protein information
Uniprot ID IL18_HUMAN
Uniprot AC Q14116; O75599; Q6FGY3; Q6WWJ7;
UniGene Hs.83077;
GeneID 3606
KEGG hsa:3606;
Human Interleukin-18 Protein SEQUENCE
SEQUENCE 193 AA; 22326 MW; 323C62C203788D55 CRC64;
MAAEPVEDNC INFVAMKFID NTLYFIAEDD ENLESDYFGK LESKLSVIRN
LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI
SVKCEKISTL SCENKIISFK EMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ
FESSSYEGYF LACEKERDLF KLILKKEDEL GDRSIMFTVQ NED
UCSC uc001pnb.2; human. [Q14116-1];

Human IL18 ELISA Kit

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