Mouse Defa2 ELISA Kit

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  • Alternative name

    Mouse Cryptdin-1 ELISA Kit;Mouse Defensin-related cryptdin-2 ELISA Kit;Mouse Defcr2 ELISA Kit;

  • Catalog
    E050652
  • species
    Mouse
  • GeneDefa2
  • Standard CurveMouse Defa2 ELISA Kit
  • Other Species Human DEFA2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Defa2 ELISA Kit allows for the in vitro quantitative determination of Defa2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: DEFA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFA2 antibody and an DEFA2-HRP conjugate. The assay sample and buffer are incubated together with DEFA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFA2 concentration since DEFA2 from samples and DEFA2-HRP conjugate compete for the anti-DEFA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFA2 from the sample, fewer sites are left to bind DEFA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFA2 concentration in each sample is interpolated from this standard curve.
  • Mouse Alpha-defensin 2 Protein information
  • Uniprot ID DEFA2_MOUSE
  • Uniprot AC P28309; P82293; Q60616; Q62537;
  • UniGene Mm.327348; Mm.425522;
  • Mouse Alpha-defensin 2 Protein SEQUENCE
  • SEQUENCE 93 AA; 10560 MW; 4F05DEA252412B0D CRC64;

    MKPLVLLSAL VLLSFQVQAD PIQNTDEETK TEEQSGEEDQ AVSVSFGDRE

    GASLQEESLR DLVCYCRTRG CKRRERMNGT CRKGHLMYTL CCR




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