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Human NUP85 ELISA Kit

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  • Alternative name

    Human 85 kDa nucleoporin ELISA Kit;Human FROUNT ELISA Kit;Human nucleoporin Nup75 ELISA Kit;Human nucleoporin Nup85 ELISA Kit;Human Pericentrin-1 ELISA Kit;Human Nup75 ELISA Kit;Human PCNT1 ELISA Kit;Human nucleoporin 85 ELISA Kit;Human nuclear pore complex protein Nup85 ELISA Kit;Human nucleoporin 85kDa ELISA Kit;
  • Catalog
    E027182
  • species
    Human
  • GeneNUP85
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NUP85. No significant cross-reactivity or interference between Human NUP85 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human NUP85 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman NUP85 ELISA Kit allows for the in vitro quantitative determination of NUP85 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NUP85 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NUP85. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human NUP85 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NUP85, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NUP85. You can calculate the concentration of Human NUP85 in the samples by comparing the OD of the samples to the standard curve.
  • Human Nuclear pore complex protein Nup85 Protein information
  • Uniprot ID NUP85_HUMAN
  • Uniprot AC Q9BW27; B4DMQ3; B4DPW1; Q8NDI4; Q9H9U1;
  • UniGene Hs.362817;
  • GeneID 79902
  • KEGG hsa:79902;
  • Human Nuclear pore complex protein Nup85 Protein SEQUENCE

  • SEQUENCE 656 AA; 75019 MW; 1AE2BA17D9CADF3D CRC64;

    MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS

    CPFIYIIRKD VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR

    VSKNYRSVIR ACMEEMHQVA IAAKDPANGR QFSSQVSILS AMELIWNLCE

    ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL SADVLGSENP SKHDSFWNLV

    TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM PILSPGNTQT

    LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL

    LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL

    AAFEFDIHQV IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE

    FLLLEYASGL FAHPSLWQLG VDYFDYCPEL GRVSLELHIE RIPLNTEQKA

    LKVLRICEQR QMTEQVRSIC KILAMKAVRN NRLGSALSWS IRAKDAAFAT

    LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK YREFHRMYGE

    KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL

    MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE

    GSLEGS

  • UCSC uc002jng.2; human. [Q9BW27-1];



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