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Human SEMA4B ELISA Kit

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  • Alternative name

    Human KIAA1745 ELISA Kit;Human SEMAC ELISA Kit;Human SemC ELISA Kit;Human semaphorin 4B ELISA Kit;Human semaphorin-4B ELISA Kit;Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B ELISA Kit;Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B ELISA Kit;
  • Catalog
    E034601
  • species
    Human
  • GeneSEMA4B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SEMA4B. No significant cross-reactivity or interference between SEMA4B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SEMA4B is typically less than 0.071ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by ad
  • Intended UseHuman SEMA4B ELISA Kit allows for the in vitro quantitative determination of SEMA4B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA4B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SEMA4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA4B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Semaphorin-4B Protein information
  • Uniprot ID SEM4B_HUMAN
  • Uniprot AC Q9NPR2; Q6UXE3; Q8WVP9; Q96FK5; Q9C0B8; Q9H691; Q9NPM8; Q9NPN0;
  • UniGene Hs.474935;
  • GeneID 10509
  • KEGG hsa:10509;
  • Human Semaphorin-4B Protein SEQUENCE

  • SEQUENCE 832 AA; 92193 MW; 29D58C1DDD5E1C6B CRC64;

    MGLRSWLAAP WGALPPRPPL LLLLLLLLLL QPPPPTWALS PRISLPLGSE

    ERPFLRFEAE HISNYTALLL SRDGRTLYVG AREALFALSS NLSFLPGGEY

    QELLWGADAE KKQQCSFKGK DPQRDCQNYI KILLPLSGSH LFTCGTAAFS

    PMCTYINMEN FTLARDEKGN VLLEDGKGRC PFDPNFKSTA LVVDGELYTG

    TVSSFQGNDP AISRSQSLRP TKTESSLNWL QDPAFVASAY IPESLGSLQG

    DDDKIYFFFS ETGQEFEFFE NTIVSRIARI CKGDEGGERV LQQRWTSFLK

    AQLLCSRPDD GFPFNVLQDV FTLSPSPQDW RDTLFYGVFT SQWHRGTTEG

    SAVCVFTMKD VQRVFSGLYK EVNRETQQWY TVTHPVPTPR PGACITNSAR

    ERKINSSLQL PDRVLNFLKD HFLMDGQVRS RMLLLQPQAR YQRVAVHRVP

    GLHHTYDVLF LGTGDGRLHK AVSVGPRVHI IEELQIFSSG QPVQNLLLDT

    HRGLLYAASH SGVVQVPMAN CSLYRSCGDC LLARDPYCAW SGSSCKHVSL

    YQPQLATRPW IQDIEGASAK DLCSASSVVS PSFVPTGEKP CEQVQFQPNT

    VNTLACPLLS NLATRLWLRN GAPVNASASC HVLPTGDLLL VGTQQLGEFQ

    CWSLEEGFQQ LVASYCPEVV EDGVADQTDE GGSVPVIIST SRVSAPAGGK

    ASWGADRSYW KEFLVMCTLF VLAVLLPVLF LLYRHRNSMK VFLKQGECAS

    VHPKTCPVVL PPETRPLNGL GPPSTPLDHR GYQSLSDSPP GSRVFTESEK

    RPLSIQDSFV EVSPVCPRPR VRLGSEIRDS VV




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