Intended UseHuman NEB ELISA Kit allows for the in vitro quantitative determination of NEB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human NEB. During the reaction,Human NEB in the sample or standard competes with a fixed amount of Human NEB on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human NEB. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human NEB in the samples is then determined by comparing the O.D. of the samples to the standard curve.