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  • Alternative name

    Tryptophan 2,3-dioxygenase ELISA KIT; Tryptamin 2,3-dioxygenase ELISA KIT; Tryptophan oxygenase ELISA KIT; TO ELISA KIT; TRPO ELISA KIT; Tryptophan pyrrolase ELISA KIT; TryptophanaseTDO2 ELISA KIT; TDO ELISA KIT; TDO ELISA KIT; TO ELISA KIT; TRPO ELISA KIT

  • Catalog
  • species
  • GeneTDO
  • Standard CurveMouse TDO ELISA Kit
  • Other Species Human TDO ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TDO. No significant cross-reactivity or interference between TDO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TDO and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse TDO ELISA Kit allows for the in vitro quantitative determination of TDO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This TDO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TDO. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: TDO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TDO antibody and an TDO-HRP conjugate. The assay sample and buffer are incubated together with TDO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TDO concentration since TDO from samples and TDO-HRP conjugate compete for the anti-TDO antibody binding site. Since the number of sites is limited, as more sites are occupied by TDO from the sample, fewer sites are left to bind TDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDO concentration in each sample is interpolated from this standard curve.

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