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Human SLC11A1 ELISA Kit

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  • Alternative name

    Human NRAMP 1 ELISA Kit;Human solute carrier family 11 member 1 ELISA Kit;Human LSH ELISA Kit;Human NRAMP ELISA Kit;Human NRAMP1 ELISA Kit;Human natural resistance-associated macrophage protein 1 ELISA Kit;Human solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1 ELISA Kit;Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 ELISA Kit;Human solute carrier family 11 (sodium/phosphate symporters), member 1 ELISA Kit;
  • Catalog
    E025881
  • species
    Human
  • GeneSLC11A1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SLC11A1. No significant cross-reactivity or interference between SLC11A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC11A1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SLC11A1 ELISA Kit allows for the in vitro quantitative determination of SLC11A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Priciple of the assay: SLC11A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC11A1 antibody and an SLC11A1-HRP conjugate. The assay sample and buffer are incubated together with SLC11A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC11A1 concentration since SLC11A1 from samples and SLC11A1-HRP conjugate compete for the anti-SLC11A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC11A1 from the sample, fewer sites are left to bind SLC11A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC11A1 concentration in each sample is interpolated from this standard curve.
  • Human Natural resistance-associated macrophage protein 1 Protein information
  • Uniprot ID NRAM1_HUMAN
  • Uniprot AC P49279; C0H5Y3;
  • UniGene Hs.591607;
  • GeneID 6556
  • KEGG hsa:6556;
  • Human Natural resistance-associated macrophage protein 1 Protein SEQUENCE

  • SEQUENCE 550 AA; 59872 MW; AA670763D52EEECC CRC64;

    MTGDKGPQRL SGSSYGSISS PTSPTSPGPQ QAPPRETYLS EKIPIPDTKP

    GTFSLRKLWA FTGPGFLMSI AFLDPGNIES DLQAGAVAGF KLLWVLLWAT

    VLGLLCQRLA ARLGVVTGKD LGEVCHLYYP KVPRTVLWLT IELAIVGSDM

    QEVIGTAIAF NLLSAGRIPL WGGVLITIVD TFFFLFLDNY GLRKLEAFFG

    LLITIMALTF GYEYVVARPE QGALLRGLFL PSCPGCGHPE LLQAVGIVGA

    IIMPHNIYLH SALVKSREID RARRADIREA NMYFLIEATI ALSVSFIINL

    FVMAVFGQAF YQKTNQAAFN ICANSSLHDY AKIFPMNNAT VAVDIYQGGV

    ILGCLFGPAA LYIWAIGLLA AGQSSTMTGT YAGQFVMEGF LRLRWSRFAR

    VLLTRSCAIL PTVLVAVFRD LRDLSGLNDL LNVLQSLLLP FAVLPILTFT

    SMPTLMQEFA NGLLNKVVTS SIMVLVCAIN LYFVVSYLPS LPHPAYFGLA

    ALLAAAYLGL STYLVWTCCL AHGATFLAHS SHHHFLYGLL EEDQKGETSG

  • UCSC uc002vhv.3; human. [P49279-1];



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