Human GP5 ELISA Kit

Alternative name
Human GPV ELISA Kit;Human glycoprotein 5 ELISA Kit;Human CD42d ELISA Kit;Human glycoprotein V platelet ELISA Kit;Human platelet glycoprotein V ELISA Kit;
Catalog
E017154
species
Human
GeneGP5
Standard Curve
Other Species Mouse Gp5 ELISA Kit Rat Gp5 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of PCL5. No significant cross-reactivity or interference between PCL5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCL5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GP5 ELISA Kit allows for the in vitro quantitative determination of GP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: PCL5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PCL5. Standards or samples are then added to the microtiter plate wells and PCL5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PCL5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PCL5 are added to each well to "sandwich" the PCL5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PCL5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCL5 concentration in each sample is interpolated from this standard curve.
Human Platelet glycoprotein V Protein information
Uniprot ID GPV_HUMAN
Uniprot AC P40197; D1MER9;
UniGene Hs.73734;
GeneID 2814
KEGG hsa:2814;
Human Platelet glycoprotein V Protein SEQUENCE
SEQUENCE 560 AA; 60959 MW; B1CDB04AF8AF7115 CRC64;
MLRGTLLCAV LGLLRAQPFP CPPACKCVFR DAAQCSGGDV ARISALGLPT
NLTHILLFGM GRGVLQSQSF SGMTVLQRLM ISDSHISAVA PGTFSDLIKL
KTLRLSRNKI THLPGALLDK MVLLEQLFLD HNALRGIDQN MFQKLVNLQE
LALNQNQLDF LPASLFTNLE NLKLLDLSGN NLTHLPKGLL GAQAKLERLL
LHSNRLVSLD SGLLNSLGAL TELQFHRNHI RSIAPGAFDR LPNLSSLTLS
RNHLAFLPSA LFLHSHNLTL LTLFENPLAE LPGVLFGEMG GLQELWLNRT
QLRTLPAAAF RNLSRLRYLG VTLSPRLSAL PQGAFQGLGE LQVLALHSNG
LTALPDGLLR GLGKLRQVSL RRNRLRALPR ALFRNLSSLE SVQLDHNQLE
TLPGDVFGAL PRLTEVLLGH NSWRCDCGLG PFLGWLRQHL GLVGGEEPPR
CAGPGAHAGL PLWALPGGDA ECPGPRGPPP RPAADSSSEA PVHPALAPNS
SEPWVWAQPV TTGKGQDHSP FWGFYFLLLA VQAMITVIIV FAMIKIGQLF
RKLIRERALG
UCSC uc062rou.1; human.;

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Human GP5 ELISA Kit

Human GPV ELISA Kit;Human glycoprotein 5 ELISA Kit;Human CD42d ELISA Kit;Human glycoprotein V platelet ELISA Kit;Human platelet glycoprotein V ELISA Kit;