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Human PTPRO ELISA Kit

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  • Alternative name

    Human R-PTP-O ELISA Kit;Human glomerular epithelial protein 1 ELISA Kit;Human Protein tyrosine phosphatase U2 ELISA Kit;Human PTP-U2 ELISA Kit;Human PTPase U2 ELISA Kit;Human GLEPP1 ELISA Kit;Human PTPU2 ELISA Kit;Human NPHS6 ELISA Kit;Human PTP-OC ELISA Kit;Human PTPROT ELISA Kit;Human protein tyrosine phosphatase, receptor type O ELISA Kit;Human receptor-type tyrosine-protein phosphatase O ELISA Kit;Human PTP phi ELISA Kit;Human osteoclastic transmembrane protein-tyrosine phosphatase ELISA Kit;Human phosphotyrosine phosphatase U2 ELISA Kit;Human protein tyrosine phosphatase PTP-U2 ELISA Kit;
  • Catalog
    E031857
  • species
    Human
  • GenePTPRO
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTPRO. No significant cross-reactivity or interference between PTPRO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTPRO and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PTPRO ELISA Kit allows for the in vitro quantitative determination of PTPRO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: PTPRO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTPRO antibody and an PTPRO-HRP conjugate. The assay sample and buffer are incubated together with PTPRO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTPRO concentration since PTPRO from samples and PTPRO-HRP conjugate compete for the anti-PTPRO antibody binding site. Since the number of sites is limited, as more sites are occupied by PTPRO from the sample, fewer sites are left to bind PTPRO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTPRO concentration in each sample is interpolated from this standard curve.
  • Human Receptor-type tyrosine-protein phosphatase O Protein information
  • Uniprot ID PTPRO_HUMAN
  • Uniprot AC Q16827; A0AV39; Q13101; Q8IYG3; Q9UBF0; Q9UBT5;
  • UniGene Hs.160871;
  • GeneID 5800
  • KEGG hsa:5800;
  • Human Receptor-type tyrosine-protein phosphatase O Protein SEQUENCE

  • SEQUENCE 1216 AA; 138344 MW; C902B48D1A73BFE1 CRC64;

    MGHLPTGIHG ARRLLPLLWL FVLFKNATAF HVTVQDDNNI VVSLEASDVI

    SPASVYVVKI TGESKNYFFE FEEFNSTLPP PVIFKASYHG LYYIITLVVV

    NGNVVTKPSR SITVLTKPLP VTSVSIYDYK PSPETGVLFE IHYPEKYNVF

    TRVNISYWEG KDFRTMLYKD FFKGKTVFNH WLPGMCYSNI TFQLVSEATF

    NKSTLVEYSG VSHEPKQHRT APYPPQNISV RIVNLNKNNW EEQSGNFPEE

    SFMRSQDTIG KEKLFHFTEE TPEIPSGNIS SGWPDFNSSD YETTSQPYWW

    DSASAAPESE DEFVSVLPME YENNSTLSET EKSTSGSFSF FPVQMILTWL

    PPKPPTAFDG FHIHIEREEN FTEYLMVDEE AHEFVAELKE PGKYKLSVTT

    FSSSGSCETR KSQSAKSLSF YISPSGEWIE ELTEKPQHVS VHVLSSTTAL

    MSWTSSQENY NSTIVSVVSL TCQKQKESQR LEKQYCTQVN SSKPIIENLV

    PGAQYQVVIY LRKGPLIGPP SDPVTFAIVP TGIKDLMLYP LGPTAVVLSW

    TRPYLGVFRK YVVEMFYFNP ATMTSEWTTY YEIAATVSLT ASVRIANLLP

    AWYYNFRVTM VTWGDPELSC CDSSTISFIT APVAPEITSV EYFNSLLYIS

    WTYGDDTTDL SHSRMLHWMV VAEGKKKIKK SVTRNVMTAI LSLPPGDIYN

    LSVTACTERG SNTSMLRLVK LEPAPPKSLF AVNKTQTSVT LLWVEEGVAD

    FFEVFCQQVG SSQKTKLQEP VAVSSHVVTI SSLLPATAYN CSVTSFSHDS

    PSVPTFIAVS TMVTEMNPNV VVISVLAILS TLLIGLLLVT LIILRKKHLQ

    MARECGAGTF VNFASLERDG KLPYNWRRSI FAFLTLLPSC LWTDYLLAFY

    INPWSKNGLK KRKLTNPVQL DDFDAYIKDM AKDSDYKFSL QFEELKLIGL

    DIPHFAADLP LNRCKNRYTN ILPYDFSRVR LVSMNEEEGA DYINANYIPG

    YNSPQEYIAT QGPLPETRND FWKMVLQQKS QIIVMLTQCN EKRRVKCDHY

    WPFTEEPIAY GDITVEMISE EEQDDWACRH FRINYADEMQ DVMHFNYTAW

    PDHGVPTANA AESILQFVHM VRQQATKSKG PMIIHCSAGV GRTGTFIALD

    RLLQHIRDHE FVDILGLVSE MRSYRMSMVQ TEEQYIFIHQ CVQLMWMKKK

    QQFCISDVIY ENVSKS

  • UCSC uc001rcu.3; human. [Q16827-1];



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