Intended UseHuman ZG16 ELISA Kit allows for the in vitro quantitative determination of ZG16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZG16. During the reaction,Human ZG16 in the sample or standard competes with a fixed amount of biotin-labeled Human ZG16 for sites on a pre-coated Monoclonal antibody specific to Human ZG16. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ZG16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Zymogen granule membrane protein 16 Protein information
Uniprot ID
ZG16_HUMAN
Uniprot AC
O60844; B2R4Z3; B9EK72;
UniGene
Hs.632195;
GeneID
653808
KEGG
hsa:653808;
Human Zymogen granule membrane protein 16 Protein SEQUENCE