Human CDK4 ELISA Kit

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  • Alternative name

    Human cell division protein kinase 4 ELISA Kit;Human PSK-J3 ELISA Kit;Human CMM3 ELISA Kit;Human cyclin dependent kinase 4 ELISA Kit;Human cyclin-dependent kinase 4 ELISA Kit;

  • Catalog
    E007590
  • species
    Human
  • GeneCDK4
  • Other Species Mouse Cdk4 ELISA KitBovine CDK4 ELISA KitPorcine CDK4 ELISA KitRat Cdk4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CDK4. No significant cross-reactivity or interference between Human CDK4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CDK4 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseHuman CDK4 ELISA Kit allows for the in vitro quantitative determination of CDK4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK4 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CDK4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK4. You can calculate the concentration of Human CDK4 in the samples by comparing the OD of the samples to the standard curve.
  • Human Cyclin-dependent kinase 4 Protein information
  • Uniprot ID CDK4_HUMAN
  • Uniprot AC P11802; B2R9A0; B4DNF9; O00576; Q6FG61;
  • UniGene Hs.95577;
  • GeneID 1019
  • KEGG hsa:1019;
  • Human Cyclin-dependent kinase 4 Protein SEQUENCE
  • SEQUENCE 303 AA; 33730 MW; 0916A0C07403A33A CRC64;

    MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP

    ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL

    RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS

    GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV

    GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP

    PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG

    NPE

  • UCSC uc001spv.4; human. [P11802-1];



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