Human IL-26 ELISA Kit;Human Protein AK155 ELISA Kit;Human AK155 ELISA Kit;Human interleukin 26 ELISA Kit;Human interleukin-26 ELISA Kit;
Catalog
E020664
species
Human
GeneIL26
Standard Curve
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 26 (IL26).
No significant cross-reactivity or interference between Interleukin 26 (IL26) and analogues was observed.
Intended UseHuman IL26 ELISA Kit allows for the in vitro quantitative determination of IL26 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 26 (IL26). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 26 (IL26). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 26 (IL26), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 26 (IL26) in the samples is then determined by comparing the O.D. of the samples to the standard curve.