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Human CNTF ELISA Kit

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  • Alternative name

    Human CNTF ELISA Kit;Human HCNTF ELISA Kit;Human ciliary neurotrophic factor ELISA Kit;
  • Catalog
    E009462
  • species
    Human
  • GeneCNTF
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CNTF. No significant cross-reactivity or interference between human CNTF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CNTF is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman CNTF ELISA Kit allows for the in vitro quantitative determination of CNTF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CNTF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CNTF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CNTF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Ciliary neurotrophic factor Protein information
  • Uniprot ID CNTF_HUMAN
  • Uniprot AC P26441; B2RAB2;
  • UniGene Hs.524920;
  • GeneID 1270
  • KEGG hsa:1270;
  • Human Ciliary neurotrophic factor Protein SEQUENCE

  • SEQUENCE 200 AA; 22931 MW; EDD9D895BDA6F35A CRC64;

    MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL

    DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP

    TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG

    LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

  • UCSC uc001nna.5; human.;



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