Mouse Ddost ELISA Kit

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  • Alternative name

    Mouse DDOST 48 kDa subunit ELISA Kit;Mouse oligosaccharyl transferase 48 kDa subunit ELISA Kit;Mouse dolichyl-di-phosphooligosaccharide-protein glycotransferase ELISA Kit;Mouse dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit;

  • Catalog
    E051203
  • species
    Mouse
  • GeneDdost
  • Standard CurveMouse Ddost ELISA Kit
  • Other Species Human DDOST ELISA KitBovine DDOST ELISA KitCanine DDOST ELISA KitChicken DDOST ELISA KitPorcine DDOST ELISA KitRat Ddost ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DDOST. No significant cross-reactivity or interference between DDOST and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of DDOST is typically less than 0.064ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseMouse Ddost ELISA Kit allows for the in vitro quantitative determination of Ddost , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDOST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DDOST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDOST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DDOST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein information
  • Uniprot ID OST48_MOUSE
  • Uniprot AC O54734; Q8C4P7;
  • UniGene Mm.470295; Mm.7236;
  • GeneID 13200
  • KEGG mmu:13200;
  • Mouse Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein SEQUENCE
  • SEQUENCE 441 AA; 49028 MW; C1B9A0B94D2A1CAC CRC64;

    MKMDPRLAVR AWPLCGLLLA VLGCVCASGP RTLVLLDNLN VRDTHSLFFR

    SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET

    ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS

    DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL

    TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS

    DAFFNSAVQK ATPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR

    VGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR

    TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH

    TQYERFIPSA YPYYASAFSM MAGLFIFSIV FLHMKEKEKS D

  • UCSC uc008vkt.1; mouse.;


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