Human GRB14 ELISA Kit

Alternative name
Human GRB14 adapter protein ELISA Kit;Human growth factor receptor bound protein 14 ELISA Kit;Human growth factor receptor-bound protein 14 ELISA Kit;
Catalog
E017456
species
Human
GeneGRB14
Other Species Mouse Grb14 ELISA Kit Bovine GRB14 ELISA Kit Rat Grb14 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human Grb14. No significant cross-reactivity or interference between Human Grb14 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human Grb14 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GRB14 ELISA Kit allows for the in vitro quantitative determination of GRB14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Grb14 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Grb14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human Grb14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Grb14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Grb14. You can calculate the concentration of Human Grb14 in the samples by comparing the OD of the samples to the standard curve.
Human Growth factor receptor-bound protein 14 Protein information
Uniprot ID GRB14_HUMAN
Uniprot AC Q14449; B7Z7F9; Q7Z6I1;
UniGene Hs.411881;
GeneID 2888
KEGG hsa:2888;
Human Growth factor receptor-bound protein 14 Protein SEQUENCE
SEQUENCE 540 AA; 60988 MW; 00472988650F7C6C CRC64;
MTTSLQDGQS AASRAAARDS PLAAQVCGAA QGRGDAHDLA PAPWLHARAL
LPLPDGTRGC AADRRKKKDL DVPEMPSIPN PFPELCCSPF TSVLSADLFP
KANSRKKQVI KVYSEDETSR ALDVPSDITA RDVCQLLILK NHYIDDHSWT
LFEHLPHIGV ERTIEDHELV IEVLSNWGIE EENKLYFRKN YAKYEFFKNP
MYFFPEHMVS FATETNGEIS PTQILQMFLS SSTYPEIHGF LHAKEQGKKS
WKKIYFFLRR SGLYFSTKGT SKEPRHLQFF SEFGNSDIYV SLAGKKKHGA
PTNYGFCFKP NKAGGPRDLK MLCAEEEQSR TCWVTAIRLL KYGMQLYQNY
MHPYQGRSGC SSQSISPMRS ISENSLVAMD FSGQKSRVIE NPTEALSVAV
EEGLAWRKKG CLRLGTHGSP TASSQSSATN MAIHRSQPWF HHKISRDEAQ
RLIIQQGLVD GVFLVRDSQS NPKTFVLSMS HGQKIKHFQI IPVEDDGEMF
HTLDDGHTRF TDLIQLVEFY QLNKGVLPCK LKHYCARIAL
UCSC uc002ucl.4; human. [Q14449-1];

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Human GRB14 ELISA Kit

Human GRB14 adapter protein ELISA Kit;Human growth factor receptor bound protein 14 ELISA Kit;Human growth factor receptor-bound protein 14 ELISA Kit;