Human SHP ELISA Kit

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  • Alternative name

    Nuclear receptor subfamily 0 group B member 2 ELISA KIT; Orphan nuclear receptor SHP ELISA KIT; Small heterodimer partnerNR0B2 ELISA KIT; SHP ELISA KIT

  • Catalog
    E035474
  • species
    Human
  • GeneSHP
  • Standard CurveHuman SHP ELISA Kit
  • Other Species Human SHP 2 ELISA KitHuman SHP-2 ELISA KitMouse shp-2 ELISA KitMouse SHP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Small Heterodimer Partner (SHP). No significant cross-reactivity or interference between Small Heterodimer Partner (SHP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman SHP ELISA Kit allows for the in vitro quantitative determination of SHP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Heterodimer Partner (SHP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Heterodimer Partner (SHP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Heterodimer Partner (SHP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Heterodimer Partner (SHP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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