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Alternative name
Platelet basic protein ELISA KIT; C-X-C motif chemokine 7 ELISA KIT; Leukocyte-derived growth factor ELISA KIT; LDGF ELISA KIT; Macrophage-derived growth factor ELISA KIT; MDGF ELISA KIT; Small-inducible cytokine B7Cleaved into the following 10 chains:Connective tissue-activating peptide III ELISA KIT; CTAP-IIIAlternative name(s):LA-PF4 ELISA KIT; Low-affinity platelet factor IVPPBP ELISA KIT; CTAP3 ELISA KIT; CXCL7 ELISA KIT; SCYB7 ELISA KIT; TGB1 ELISA KIT; THBGB1 ELISA KIT; PBP ELISA KIT; LDGF ELISA KIT; MDGF ELISA KIT; CTAP-III ELISA KIT; CTAP-III(1-81) ELISA KIT; Beta-TG ELISA KIT; NAP-2(74) ELISA KIT; NAP-2(73) ELISA KIT; NAP-2 ELISA KIT; NAP-2(1-66) ELISA KIT; NAP-2(1-63) ELISA KIT
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Catalog
E010468
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species
Human
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GeneCXCL7
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Other Species
Mouse NAP-2/CXCL7 ELISA Kit
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SpecificityNatural and recombinant human CXCL7
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 2pg/ml
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Intended UseHuman CXCL7 ELISA Kit allows for the in vitro quantitative determination of CXCL7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards (E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
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