Mouse APAF-1 ELISA Kit

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  • Alternative name

    Apoptotic protease-activating factor 1 ELISA KIT; APAF1 ELISA KIT; KIAA0413 ELISA KIT; APAF-1 ELISA KIT

  • Catalog
    E044679
  • species
    Mouse
  • GeneAPAF-1
  • Standard CurveMouse APAF-1 ELISA Kit
  • Other Species Human Apaf-1 ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity5 pg/ml.
  • Intended UseMouse APAF-1 ELISA Kit allows for the in vitro quantitative determination of APAF-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse APAF-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.



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