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Human MCM5 ELISA Kit

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  • Alternative name

    Human CDC46 homolog ELISA Kit;Human P1-CDC46 ELISA Kit;Human CDC46 ELISA Kit;Human minichromosome maintenance complex component 5 ELISA Kit;Human DNA replication licensing factor MCM5 ELISA Kit;Human MCM5 minichromosome maintenance deficient 5, cell division cycle 46 ELISA Kit;Human minichromosome maintenance deficient 5 (cell division cycle 46) ELISA Kit;
  • Catalog
    E024583
  • species
    Human
  • GeneMCM5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 5 (MCM5). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 5 (MCM5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman MCM5 ELISA Kit allows for the in vitro quantitative determination of MCM5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 5 (MCM5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 5 (MCM5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 5 (MCM5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA replication licensing factor MCM5 Protein information
  • Uniprot ID MCM5_HUMAN
  • Uniprot AC P33992; O60785; Q14578; Q9BTJ4; Q9BWL8;
  • UniGene Hs.517582;
  • GeneID 4174
  • KEGG hsa:4174;
  • Human DNA replication licensing factor MCM5 Protein SEQUENCE

  • SEQUENCE 734 AA; 82286 MW; A80280E61749998D CRC64;

    MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG

    FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLYK QPAEHLQLLE

    EAAKEVADEV TRPRPSGEEV LQDIQVMLKS DASPSSIRSL KSDMMSHLVK

    IPGIIIAASA VRAKATRISI QCRSCRNTLT NIAMRPGLEG YALPRKCNTD

    QAGRPKCPLD PYFIMPDKCK CVDFQTLKLQ ELPDAVPHGE MPRHMQLYCD

    RYLCDKVVPG NRVTIMGIYS IKKFGLTTSR GRDRVGVGIR SSYIRVLGIQ

    VDTDGSGRSF AGAVSPQEEE EFRRLAALPN VYEVISKSIA PSIFGGTDMK

    KAIACLLFGG SRKRLPDGLT RRGDINLLML GDPGTAKSQL LKFVEKCSPI

    GVYTSGKGSS AAGLTASVMR DPSSRNFIME GGAMVLADGG VVCIDEFDKM

    REDDRVAIHE AMEQQTISIA KAGITTTLNS RCSVLAAANS VFGRWDETKG

    EDNIDFMPTI LSRFDMIFIV KDEHNEERDV MLAKHVITLH VSALTQTQAV

    EGEIDLAKLK KFIAYCRVKC GPRLSAEAAE KLKNRYIIMR SGARQHERDS

    DRRSSIPITV RQLEAIVRIA EALSKMKLQP FATEADVEEA LRLFQVSTLD

    AALSGTLSGV EGFTSQEDQE MLSRIEKQLK RRFAIGSQVS EHSIIKDFTK

    QKYPEHAIHK VLQLMLRRGE IQHRMQRKVL YRLK

  • UCSC uc003anu.5; human.;



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