Human PGK1 ELISA Kit

Alternative name
Human cell migration-inducing gene 10 protein ELISA Kit;Human primer recognition protein 2 ELISA Kit;Human PRP 2 ELISA Kit;Human PGKA ELISA Kit;Human HEL-S-68p ELISA Kit;Human MIG10 ELISA Kit;Human phosphoglycerate kinase 1 ELISA Kit;Human epididymis secretory sperm binding protein Li 68p ELISA Kit;
Catalog
E029094
species
Human
GenePGK1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphoglycerate Kinase 1 (PGK1). No significant cross-reactivity or interference between Phosphoglycerate Kinase 1 (PGK1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 12.9pg/mL.
Intended UseHuman PGK1 ELISA Kit allows for the in vitro quantitative determination of PGK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate Kinase 1 (PGK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoglycerate Kinase 1 (PGK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoglycerate Kinase 1 (PGK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoglycerate Kinase 1 (PGK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Phosphoglycerate kinase 1 Protein information
Uniprot ID PGK1_HUMAN
Uniprot AC P00558; A8K4W6; B7Z7A9; Q5J7W1; Q6IBT6; Q8NI87;
UniGene Hs.78771;
GeneID 5230
KEGG hsa:5230;
Human Phosphoglycerate kinase 1 Protein SEQUENCE
SEQUENCE 417 AA; 44615 MW; B5DFC7B5FA01767C CRC64;
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC
LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV
GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR
GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
ELLEGKVLPG VDALSNI

Category

Human PGK1 ELISA Kit