Human Orexins ELISA Kit

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  • Alternative name

    Orexin ELISA KIT; Hypocretin ELISA KIT; HcrtHCRT ELISA KIT; OX ELISA KIT; PPORX ELISA KIT; PPOX ELISA KIT; Hcrt ELISA KIT; Hcrt1 ELISA KIT; Hcrt2 ELISA KIT

  • Catalog
    E027765
  • species
    Human
  • GeneOrexins
  • Other Species Mouse Orexins ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman Orexins ELISA Kit allows for the in vitro quantitative determination of Orexins , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This Orexins ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Orexins. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY Orexins ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Orexins antibody and an Orexins-HRP conjugate. The assay sample and buffer are incubated together with Orexins-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Orexins concentration since Orexins from samples and Orexins-HRP conjugate compete for the anti-Orexins antibody binding site. Since the number of sites is limited, as more sites are occupied by Orexins from the sample, fewer sites are left to bind Orexins-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Orexins concentration in each sample is interpolated from this standard curve.



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