The things you and the mice did in those years

To do experiments, we often deal with rats!

Would you not have the heart to kill a cute mouse?

For great scientific research, the mice had to be dedicated.

As one of the laboratory's common skills, mice have been widely used in various bioinduced experiments. The successful induction of drugs is half the success.

To be a qualified lab old driver, to establish an undefeated field between the mice and the mice, the basic abdominal injection and stomach operation is standard!

What is abdominal injection, what is to fill the stomach to give medicine?

Intraperitoneal injection (IP) as a kind of commonly used dosing methods, convenient, easy to absorb quickly, generally used in experiment of anesthesia, drug induced mice and commonly used in inappropriate or via the gastrointestinal tract absorption efficiency is low, and also to induce pups.

The mouse irrigation (IG) was used as another way to induce the drug, but only for adult mice.

And intraperitoneal injection of the same, the induction efficiency of highly efficient, drug after gastrointestinal absorption was transferred to the whole body, so in pregnant rat embryos can better guarantee the fetal rats induced absorption dose uniformity.

It is necessary to insert the stomach needle into the mouse esophagus and stimulate the mice, which can lead to the miscarriage of the pregnant rats, so it is usually operated under anesthesia.

It is more difficult to operate the stomach, the above is the actual drawing

• Intraperitoneal injection (IP)

Operation steps:

1.Fetching: left hand picked up the mice in from the tail, and then its contact with cage frame, due to the instinct to escape behavior in mice, it will forward to climb, the mice body, then the right hand thumb and index finger quickly and hold as much as possible edge on mice skin, also can use the middle finger and ring finger further back the rest of the region in mice skin further fixed pressure in the hand palm.

As shown in figure a.

2.Total control: grab after the mouse rollover to belly up, with the little finger mouse tail pressing on the palm of your hand, then left hind leg in mice between on the little finger and ring finger, the mice will be full control.

As shown in figure b

3.Injection: tilt the mouse head down, the internal organs of the abdominal cavity slide into the chest due to gravity, and then the lower left abdominal cavity is larger, which is the best injection site.

Into the needle cannot too deep, feel obvious sense of penetration, with extra into viscera, attention back to the suction after into needle bolt, withdraw more difficult and only be back to the suction air into the needle, success can be carried out in accordance with the need to dose injection.

Note: it is necessary to wipe the needle with alcohol or iodine before and after injection.

Note: the above operation is applicable to the mice induced by P14 and above. For the injection of intraperitoneal injection of mice below P14, the following two points should be noted in operation

1.Grasp: gently fixed the head of the mouse with the forefinger and thumb, with the rest of the fingers and hands fixed to the trunk of the mouse.

2.Injection: flip mice after belly up in the left lower abdomen into the needle and injection speed slower, and after the injection can't immediately pull out the needle, needle to spend some time in the abdominal cavity, blocking outflows, the wound to stop drugs make drugs with viscera peristalsis moved.

• Stomach (IG)

Steps:

1.Anaesthesia: isoflurane anaesthesia, after the mouse coma, observe its respiration rate, breathing evenly, slow can take out the next operation.

2.Control mice: grasp and control operation with abdominal injection

3.Lavage: keep the mice head and neck into a straight line, convenient filling and needle into the stomach, gastric needles from mice into the corner of his mouth, tongue down, hold onto the palate, gently push inward, after entering the esophagus will have an empty feeling, push down into the esophagus after injection liquid, push note process to slow uniform, such as injection amount is larger, should adopt the intermittent injection, the injection to continue a pause after a certain amount.

Operate the diagram.

Stomach volume: 0.1%-0.2ml / 10g, the maximum 0.35/10g, the maximum volume of each adult mouse was 0.8ml.

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