Human WNT4 ELISA Kit

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  • Alternative name

    Human SERKAL ELISA Kit;Human WNT-4 ELISA Kit;Human Wnt family member 4 ELISA Kit;Human protein Wnt-4 ELISA Kit;Human wingless-type MMTV integration site family, member 4 ELISA Kit;

  • Catalog
    E031924
  • species
    Human
  • GeneWNT4
  • Other Species Mouse Wnt4 ELISA KitChicken WNT4 ELISA KitTurkey WNT4 ELISA KitRat Wnt4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human WNT4. No significant cross-reactivity or interference between Human WNT4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human WNT4 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).
  • Intended UseHuman WNT4 ELISA Kit allows for the in vitro quantitative determination of WNT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative WNT4 in Human serum, plasma, tissue homogenates. An antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for WNT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein Wnt-4 Protein information
  • Uniprot ID WNT4_HUMAN
  • Uniprot AC P56705; B4DJF9; Q5TZQ0; Q96T81; Q9BXF5; Q9H1J8; Q9UJM2;
  • UniGene Hs.25766;
  • GeneID 54361
  • KEGG hsa:54361;
  • Human Protein Wnt-4 Protein SEQUENCE
  • SEQUENCE 351 AA; 39052 MW; 465D08755C992DA8 CRC64;

    MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI

    QRQVQMCKRN LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK

    VVTQGTREAA FVYAISSAGV AFAVTRACSS GELEKCGCDR TVHGVSPQGF

    QWSGCSDNIA YGVAFSQSFV DVRERSKGAS SSRALMNLHN NEAGRKAILT

    HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG ATEVEPRRVG

    SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDMRSGVLG TRGRTCNKTS

    KAIDGCELLC CGRGFHTAQV ELAERCSCKF HWCCFVKCRQ CQRLVELHTC

    R

  • UCSC uc001bfs.5; human. [P56705-1];


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