Human CYP1A1 ELISA Kit

Alternative name
Human CYPIA1 ELISA Kit;Human cytochrome P450 form 6 ELISA Kit;Human cytochrome P450-C ELISA Kit;Human cytochrome P450-P1 ELISA Kit;Human AHH ELISA Kit;Human AHRR ELISA Kit;Human CP11 ELISA Kit;Human CYP1 ELISA Kit;Human P1-450 ELISA Kit;Human P450-C ELISA Kit;Human P450DX ELISA Kit;Human cytochrome P450 family 1 subfamily A member 1 ELISA Kit;Human cytochrome P450 1A1 ELISA Kit;Human aryl hydrocarbon hydroxylase ELISA Kit;Human cytochrome P1-450, dioxin-inducible ELISA Kit;Human cytochrome P450, family 1, subfamily A, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011014
species
Human
GeneCYP1A1
Standard Curve
Other Species Mouse Cyp1a1 ELISA Kit Canine CYP1A1 ELISA Kit Guinea Pig CYP1A1 ELISA Kit Monkey CYP1A1 ELISA Kit Rabbit CYP1A1 ELISA Kit Rat Cyp1a1 ELISA Kit Sheep CYP1A1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A1 (CYP1A1). No significant cross-reactivity or interference between Cytochrome P450 1A1 (CYP1A1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.068ng/mL.
Intended UseHuman CYP1A1 ELISA Kit allows for the in vitro quantitative determination of CYP1A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A1 (CYP1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A1 (CYP1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A1 (CYP1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A1 (CYP1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome P450 1A1 Protein information
Uniprot ID CP1A1_HUMAN
Uniprot AC P04798; A4F3V9; A4F3W0; Q53G18;
UniGene Hs.72912;
GeneID 1543
KEGG hsa:1543;
Human Cytochrome P450 1A1 Protein SEQUENCE
SEQUENCE 512 AA; 58165 MW; 3C62366044148EFD CRC64;
MLFPISMSAT EFLLASVIFC LVFWVIRASR PQVPKGLKNP PGPWGWPLIG
HMLTLGKNPH LALSRMSQQY GDVLQIRIGS TPVVVLSGLD TIRQALVRQG
DDFKGRPDLY TFTLISNGQS MSFSPDSGPV WAARRRLAQN GLKSFSIASD
PASSTSCYLE EHVSKEAEVL ISTLQELMAG PGHFNPYRYV VVSVTNVICA
ICFGRRYDHN HQELLSLVNL NNNFGEVVGS GNPADFIPIL RYLPNPSLNA
FKDLNEKFYS FMQKMVKEHY KTFEKGHIRD ITDSLIEHCQ EKQLDENANV
QLSDEKIINI VLDLFGAGFD TVTTAISWSL MYLVMNPRVQ RKIQEELDTV
IGRSRRPRLS DRSHLPYMEA FILETFRHSS FVPFTIPHST TRDTSLKGFY
IPKGRCVFVN QWQINHDQKL WVNPSEFLPE RFLTPDGAID KVLSEKVIIF
GMGKRKCIGE TIARWEVFLF LAILLQRVEF SVPLGVKVDM TPIYGLTMKH
ACCEHFQMQL RS
UCSC uc002ayp.4; human. [P04798-1];

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Human CYP1A1 ELISA Kit