Human CNR1 ELISA Kit

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  • Alternative name

    Human CB-R ELISA Kit;Human CB1 ELISA Kit;Human CANN6 ELISA Kit;Human CNR ELISA Kit;Human CB1A ELISA Kit;Human CB1K5 ELISA Kit;Human CB1R ELISA Kit;Human cannabinoid receptor 1 ELISA Kit;Human cannabinoid receptor 1 (brain) ELISA Kit;Human central cannabinoid receptor ELISA Kit;

  • Catalog
    E005914
  • species
    Human
  • GeneCNR1
  • Standard CurveHuman CNR1 ELISA Kit
  • Other Species Mouse Cnr1 ELISA KitRat Cnr1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CNR1. No significant cross-reactivity or interference between CNR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNR1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CNR1 ELISA Kit allows for the in vitro quantitative determination of CNR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This CNR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CNR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNR1 antibody and an CNR1-HRP conjugate. The assay sample and buffer are incubated together with CNR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNR1 concentration since CNR1 from samples and CNR1-HRP conjugate compete for the anti-CNR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNR1 from the sample, fewer sites are left to bind CNR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNR1 concentration in each sample is interpolated from this standard curve.
  • Human Cannabinoid receptor 1 Protein information
  • Uniprot ID CNR1_HUMAN
  • Uniprot AC Q5UB37; Q9UNN0;
  • UniGene Hs.75110;
  • GeneID 1268
  • KEGG hsa:1268;
  • Human Cannabinoid receptor 1 Protein SEQUENCE
  • SEQUENCE 472 AA; 52858 MW; 1D2E49061D12ABF2 CRC64;

    MKSILDGLAD TTFRTITTDL LYVGSNDIQY EDIKGDMASK LGYFPQKFPL

    TSFRGSPFQE KMTAGDNPQL VPADQVNITE FYNKSLSSFK ENEENIQCGE

    NFMDIECFMV LNPSQQLAIA VLSLTLGTFT VLENLLVLCV ILHSRSLRCR

    PSYHFIGSLA VADLLGSVIF VYSFIDFHVF HRKDSRNVFL FKLGGVTASF

    TASVGSLFLT AIDRYISIHR PLAYKRIVTR PKAVVAFCLM WTIAIVIAVL

    PLLGWNCEKL QSVCSDIFPH IDETYLMFWI GVTSVLLLFI VYAYMYILWK

    AHSHAVRMIQ RGTQKSIIIH TSEDGKVQVT RPDQARMDIR LAKTLVLILV

    VLIICWGPLL AIMVYDVFGK MNKLIKTVFA FCSMLCLLNS TVNPIIYALR

    SKDLRHAFRS MFPSCEGTAQ PLDNSMGDSD CLHKHANNAA SVHRAAESCI

    KSTVKIAKVT MSVSTDTSAE AL





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