Specific experimental methods of chromatin immunological precipitation (Chip)

Chromatin immunisation (Chip) is a new technique widely used to identify specific proteins that bind to a particular genome area, or in turn identify the chromatin regions that bind to specific proteins.

These proteins are typically histones or proteins that bind to DNA and transcription factors.

As shown in the above, through the use of such as formaldehyde crosslinking reagent, chromatin DNA combine with proteins to form complex, 300-1000 bp forms through ultrasonic shear is equivalent to about 2-5 pieces of nucleosome, to join the antibodies to recognize the specific transcription factor, through A standard immune precipitation step, be detected antibody fragments of chromatin adsorption on the Protein - A/G beads, after washing, crosslinking and purification solutions had to be identification combined with the DNA sequence of A specific transcription factor.

So by identifying the properties of these transcription factors, the regulation of specific transcription factors under certain physiological conditions is clarified.

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