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Alternative name
Human C-C motif chemokine 11 ELISA Kit;Human eosinophil chemotactic protein ELISA Kit;Human Small-inducible cytokine A11 ELISA Kit;Human SCYA11 ELISA Kit;Human C-C motif chemokine ligand 11 ELISA Kit;Human eotaxin ELISA Kit;Human chemokine (C-C motif) ligand 11 ELISA Kit;Human eotaxin-1 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) ELISA Kit;
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Catalog
E007953
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species
Human
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GeneCCL11
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human ECF. No significant cross-reactivity or interference between human ECF and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human ECF is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
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Intended UseHuman CCL11 ELISA Kit allows for the in vitro quantitative determination of CCL11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ECF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human Eotaxin Protein information
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Uniprot ID
CCL11_HUMAN
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Uniprot AC
P51671; P50877; Q92490; Q92491;
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UniGene
Hs.54460;
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GeneID
6356
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KEGG
hsa:6356;
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Human Eotaxin Protein SEQUENCE
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SEQUENCE 97 AA; 10732 MW; B433C30FDA4C71A7 CRC64;
MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR
RITSGKCPQK
AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP
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