Human CHRM2 ELISA Kit

Alternative name
Human HM2 ELISA Kit;Human cholinergic receptor muscarinic 2 ELISA Kit;Human muscarinic acetylcholine receptor M2 ELISA Kit;Human 7TM receptor ELISA Kit;Human acetylcholine receptor, muscarinic 2 ELISA Kit;Human cholinergic receptor, muscarinic 2 ELISA Kit;Human muscarinic M2 receptor ELISA Kit;
Catalog
E025167
species
Human
GeneCHRM2
Standard Curve
Other Species Human CHRM2 Ab ELISA Kit Mouse Chrm2 ELISA Kit Mouse CHRM2 Ab ELISA Kit Bovine CHRM2 ELISA Kit Chicken CHRM2 ELISA Kit Porcine CHRM2 ELISA Kit Rat Chrm2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHRM2. No significant cross-reactivity or interference between CHRM2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHRM2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CHRM2 ELISA Kit allows for the in vitro quantitative determination of CHRM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalIntended Uses: This CHRM2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CHRM2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CHRM2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CHRM2 antibody and an CHRM2-HRP conjugate. The assay sample and buffer are incubated together with CHRM2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRM2 concentration since CHRM2 from samples and CHRM2-HRP conjugate compete for the anti-CHRM2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRM2 from the sample, fewer sites are left to bind CHRM2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRM2 concentration in each sample is interpolated from this standard curve.
Human Muscarinic acetylcholine receptor M2 Protein information
Uniprot ID ACM2_HUMAN
Uniprot AC P08172; Q4VBK6; Q9P1X9;
UniGene Hs.535891;
GeneID 1129
KEGG hsa:1129;
Human Muscarinic acetylcholine receptor M2 Protein SEQUENCE
SEQUENCE 466 AA; 51715 MW; 2FC2FD7748C22BEC CRC64;
MNNSTNSSNN SLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
IMTVLYWHIS RASKSRIKKD KKEPVANQDP VSPSLVQGRI VKPNNNNMPS
SDDGLEHNKI QNGKAPRDPV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
ITQDENTVST SLGHSKDENS KQTCIRIGTK TPKSDSCTPT NTTVEVVGSS
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
FKHLLMCHYK NIGATR
UCSC uc003vtg.2; human.;

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Human CHRM2 ELISA Kit

Human HM2 ELISA Kit;Human cholinergic receptor muscarinic 2 ELISA Kit;Human muscarinic acetylcholine receptor M2 ELISA Kit;Human 7TM receptor ELISA Kit;Human acetylcholine receptor, muscarinic 2 ELISA Kit;Human cholinergic receptor, muscarinic 2 ELISA Kit;Human muscarinic M2 receptor ELISA Kit;