Human CEP55 ELISA Kit

Alternative name
Human Cep55 ELISA Kit;Human up-regulated in colon cancer 6 ELISA Kit;Human C10orf3 ELISA Kit;Human URCC6 ELISA Kit;Human CT111 ELISA Kit;Human centrosomal protein 55 ELISA Kit;Human centrosomal protein of 55 kDa ELISA Kit;Human cancer/testis antigen 111 ELISA Kit;Human centrosomal protein 55kDa ELISA Kit;
Catalog
E007816
species
Human
GeneCEP55
SpecificityThis assay has high sensitivity and excellent specificity for detection of CEP55. No significant cross-reactivity or interference between CEP55 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CEP55 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CEP55 ELISA Kit allows for the in vitro quantitative determination of CEP55 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the Assay: CEP55 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CEP55 antibody and an CEP55-HRP conjugate. The assay sample and buffer are incubated together with CEP55-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEP55 concentration since CEP55 from samples and CEP55-HRP conjugate compete for the anti-CEP55 antibody binding site. Since the number of sites is limited, as more sites are occupied by CEP55 from the sample, fewer sites are left to bind CEP55-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEP55 concentration in each sample is interpolated from this standard curve.
Human Centrosomal protein of 55 kDa Protein information
Uniprot ID CEP55_HUMAN
Uniprot AC Q53EZ4; B2RDG8; Q32WF5; Q3MV20; Q5VY28; Q6N034; Q96H32; Q9NVS7;
UniGene Hs.14559;
GeneID 55165
KEGG hsa:55165;
Human Centrosomal protein of 55 kDa Protein SEQUENCE
SEQUENCE 464 AA; 54178 MW; A652F86EBCE46A51 CRC64;
MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK
LTDKERHRLL EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTTL
LEQLEETTRE GERREQVLKA LSEEKDVLKQ QLSAATSRIA ELESKTNTLR
LSQTVAPNCF NSSINNIHEM EIQLKDALEK NQQWLVYDQQ REVYVKGLLA
KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL LASAKKDLEV
ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK
TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA
LLEQQMQACT LDFENEKLDR QHVQHQLHVI LKELRKARNQ ITQLESLKQL
HEFAITEPLV TFQGETENRE KVAASPKSPT AALNESLVEC PKCNIQYPAT
EHRDLLVHVE YCSK
UCSC uc001kiq.4; human. [Q53EZ4-1];

Category

Human CEP55 ELISA Kit